This could contribute to the differences in tumor multiplicity and survival within this group, as other studies where serum metabolites were analyzed found increased amino-acid metabolites in ApcMin/+ mice as well as in the serum of mice bearing colon tumors of the HT-29 cell line where the APC gene was truncated.28 On the other hand, some amino-acid metabolite levels were depleted in the ApcMin/+:Thbs1−/− fed a high-fat diet when compared with ApcMin/+ mice. This evidence concerns the gene APC and neoplasm.