Moreover, ApcMin/+:Thbs1−/−mice fed a low-fat diet show elevated levels of 2HG, which is known as an oncometabolite.60 Several factors including mutations in isocitrate dehydrogenase lead to accumulation of 2HG, which is considered a poor prognostic factor in many cancers including colon cancer.61, 62 The accumulation of this metabolite is another indication of defects in metabolism that are caused by the deletion of Thbs1 in the ApcMin/+ mice and could lead to more aggressive formation of tumors in this model. This evidence concerns the gene THBS1 and cancer.