E2F2, as a member of the E2F family of transcription factors, has a crucial role in the control of the G1/S transition and DNA replication in mammalian cells.33 Overexpression of E2F2 was detected during c-myc and c-myc/TGFα-induced hepatocarcinogenesis in trans-genetic mice.34 In the present study, the deletion of ANCCA/PRO2000 inhibited HCC cell growth and cell cycle progression accompanying with the downregulation of E2F2 expression. Here, TGFA is linked to hepatocellular carcinoma.