Given the association between DENR mutations and autism spectrum disorder, a neurodevelopmental condition that is correlated with functional deficits in synaptic signaling (Chen et al., 2015), we analyzed dendritic spine density and spine morphologies of neurons in the context of Denr perturbations by combining high-power confocal microscopy with digital image reconstruction and morphometric analysis (Ngo et al., 2014; see Experimental Procedures). The gene discussed is DENR; the disease is autism spectrum disorder.