Given the relevant role of Fbxl17 in regulating Hh signaling and that Hh signaling activity has been implicated in the proliferation of prostate cancer cell lines (Karhadkar et al, 2004; Sanchez et al, 2004), we further tested the effect of Fbxl17 on Hh activation by quantifying Gli1 mRNA levels upon either depletion or expression of Fbxl17 using PC3 cells. This evidence concerns the gene FBXL17 and prostate cancer.