The expression of MMP-1 in HCC and MRC-5 co-culture model was analysed under different culture conditions: 10% FBS and O2 (10% FBS cell culture medium with O2), and with neither (without FBS and O2) to explore the effect of simulating hypoxia and starved of fetal bovine serum condition on MMP-1 secretion. The gene discussed is MMP1; the disease is hepatocellular carcinoma.