Other studies have shown that Pax6 is critical to the maintenance and regeneration of the corneal epithelium.13 Ouyang et al. showed that this is related to the role of PAX6 in facilitating the maintenance and differentiation of explanted human limbal stem cells into corneal epithelial cells.14 Indeed, heterozygous loss of PAX6 function is also associated with corneal surface disease in people with aniridia, in whom corneal opacification is frequently observed as transparent epithelial cells normally maintained by limbal stem cells are replaced by opaque conjunctiva‐like cells.31 This evidence concerns the gene PAX6 and isolated aniridia.