We analyzed the association of these CLU loci with AD related brain structures (middle temporal gyrus, posterior cingulate, precuneus, parahippocampal gyrus and hippocampus, as well as the thickness of entorhinal cortex)40, 41, 42, 43 in a model which rectified age, gender, education years, ApoE ε4 status and intracranial volume (ICV) as covariates at baseline and two-year followup study (Supplementary Table S4–S11). This evidence concerns the gene APOE and Alzheimer disease.