To test whether the rapid activation of AMPK upon matrix deprivation is cell line-specific, we took cancer cell lines from different tissues, such as breast (MCF7), cervix (HeLa S3), lung (A549), melanoma (G361) and human embryonic kidney (HEK 293T), and subjected them to detachment (suspension culture) for 10 min. The gene discussed is PRKAA2; the disease is cancer.