CCND1 and infection: Quantitative real-time RT-PCR (qRT-PCR) analysis was used to measure the mRNA expression levels of β-catenin, cyclin D1, Dvl, LRP5, or matrix metalloproteinase-7 (MMP7) after infection with RVFV MP12 or RVFV MP12-GFP (MOI of 1) or treatment with Wnt3A in A549 cells.