NOVA2 HITS-CLIP and RNA-seq analysis revealed that 11 NOVA2 target alternative splicing events on 9 genes (Dcc, Robo2, Epha5, Slit2, Ank2, Dclk1, Enah, Cask, Ptprs) intersected with the genes associated with mouse ACC phenotypes and human ACC syndromes (Figure 6—figure supplement 3), suggesting that the ACC phenotype in Nova2-/- mice may be caused by splicing dysregulation of multiple coordinately NOVA2-regulated transcripts, consistent with previous observations that NOVA proteins regulate subsets of transcripts that mediate coordinate biologic functions (Ule et al., 2005b). This evidence concerns the gene ROBO2 and ACCES syndrome.