In this study, we detected EGFR mutations in cell lines using PCR and the direct DNA sequencing technique, and the data confirmed EGFR mutations in these NSCLC cell lines that matched with the ATCC cell line characteristics, i.e., HCC-827 cells had an acquired EGFR mutation at the tyrosine kinase domain (E746-A750 deletion), while H-1975 cells harbored the heterozygous EGFR L858R mutation. Here, EGFR is linked to hepatocellular carcinoma.