To examine whether the effect of MFB on breast cancer cells might be inhibited by the expression levels of stemness-related molecules, we used immunofluorescence staining and FACS to isolate CSCs (CD44+CD24−/low) and their counterpart non-stem cancer cells (NSCs; CD44−/lowCD24+) from BT20 and HCC1937 TNBC cells. The gene discussed is CD44; the disease is breast cancer.