MTOR and breast cancer: Various breast cancer cell lines (luminal type, MCF7 and BT474; TNBC type, BT20, MDA-MB-231, Hs578T and NDY-1) were treated with metformin or MFB (10 mM) for 12 h, and cell lysates were analyzed for their levels of p-AMPK (T172) and p-mTOR (S2448).