EZH2 and osteosarcoma: To determine the migratory response of osteosarcoma cells to EZH2 knockdown, using a transwell assay, the average number of invasive cells was observed to be reduced approximately 50% and 67% after transfection with EZH2 siRNA as compared with U2OS and SAOS cells transfected with non-specific siRNA (NS) (P < 0.001 and P < 0.001, respectively) (Figure 3D, 3E).