RT-PCR assay results revealed that the demethylation of CpG-116, CpG-80, CpG+97, and CpG+102 in RNF180 DNA promoter could partially suppress the transcriptional levels of common genes related to the malignant biological characteristics of gastric cancer cells, including proliferation, invasion, angiogenesis, lymphangiogenesis, and chemotaxis. The gene discussed is RNF180; the disease is gastric cancer.