RNF180 and cancer: The mean 72 h IRs of the three other cancer cell lines transfected with the demethylated CpG island vectors (pCMV6-RNF180-DCpG-116, pCMV6-RNF180-DCpG-80, and pCMV6-RNF180-DCpG+97) were 7.22%, 2.75%, and 10.73%, respectively (Figure 4B).