To determine the mechanisms underlying MLN4924-induced inhibition, we treated SJSA-1 cells with three sub-toxic concentrations of MLN4924 (0.04, 0.2, or 1 μM) for 6, 24, or 48 h and examined the effects on neddylation of cullins and levels of tumor-suppressive CRLs substrates using western blotting analysis. The gene discussed is CACUL1; the disease is neoplasm.