To evaluate whether the neddylation pathway is activated in OS, levels of the key components NAE1 and Ubiquitin-Conjugating Enzyme E2M (Ube2M) were examined by immunohistochemical (IHC) staining analysis in a tissue array containing tumor tissues from 40 OS patients (4 stage I and 36 stage II cases). This evidence concerns the gene NAE1 and neoplasm.