To study the effect of TGFβ at an early stage of tumor development, we obtained TAs from familial adenomatous polyposis (FAP) patients, which would predictably follow the classical path of CRC development (Fearon & Vogelstein, 1990; De Sousa E Melo et al, 2013). The gene discussed is TGFB1; the disease is Familial adenomatous polyposis.