CREB1 and major depressive disorder: In this bioinformatics approach, differentially expressed genes are first identified, and the promoter DNA sequence of each differentially expressed gene is then scanned to identify the prevalence of TF-binding motifs (TFBMs) that are hypothesized to be activated in association with the condition under study (for example, MDD-associated activation of CREB/ATF TFs, which mediate signaling by many neurotransmitter systems including catecholamines from the sympathetic nervous system).