MAP1LC3A and infection: When Raw264.7, MEF, and HeLa cells were infected with P. acnes at an MOI of 1000 and examined at 1, 2, 4, 8, 16, and 24 h after infection, P. acnes were found within the autophagy marker LC3-positive vacuoles from 2 h to 24 h postinfection in all three cell lines.