ZNF582 and colorectal carcinoma: Finally, to evaluate the long-term effect of ZNF582-AS1, we performed colony-formation assays and found that ZNF582-AS1 suppressed colony formation in 2 out of 3 CRC cell lines (RKO and SW480), but it did not show a suppressive effect in HCT116 cells (Fig. 7d,e).