Using live imaging and assessment of expression of hypoxia-inducible genes we were able to detect and quantify hypoxia-related changes through the expression of carbonic anhydrase (CAIX) and the genes Pgk1, Glut1, Vegfa, Hprt, Nos2, Car9 (CAIX) and Hif1α within the lungs of mice suffering from IPA. Here, HIF1A is linked to Ito hypomelanosis.