We next determined the levels of protein expression for several examples by flow cytometry of non-separated ascites samples and confirmed the preferential expression of S100A8/A9 and IL-8 in TAMs, and of LIFR and TGFBR3 in tumor cells (Fig. 3c and Additional file 2: Figure S2). The gene discussed is IGKV1D-22; the disease is neoplasm.