CFTR and cystic fibrosis: Although CFTR sequencing is not done from the same dry-blood spot as the IRT measurement by other CF NBS models, it is a technology available for all and used post-referral in most screen-positives with one mutation detected and intermediate SC results.[35] These data can help clinicians, investigators, public health experts, and parents understand the risk of CF for children with mutations of uncertain clinical significance and impact clinical care protocols.