Through immortalization of this cell line with ts LT and hTERT, we have established the first Wilms tumor cell line with a homozygous deletion of the entire WT1 gene, resulting in a complete lack of WT1. This immortalized Wilms10 cell line should be useful for further exploration of the effect of WT1 loss in genetic and biochemical studies and to further explore the origin and cell fate of Wilms tumors with WT1 mutations. This evidence concerns the gene WT1 and Nephroblastoma.