As our results clearly showed that ANO1 surface expression is enhanced by 14-3-3γ binding, and that gene silencing of ANO1 or 14-3-3γ suppresses migration and invasion of glioblastoma cells, 14-3-3γ-mediated ANO1 surface expression may play a key role in tumorigenesis by activating MAPK signaling or NF-κB signaling, which should be investigated in a future study. The gene discussed is YWHAG; the disease is glioblastoma.