Comparing the distinct isotypes of the recommended aPL detected by LIA and ELISA in 161 patients with APS and 156 controls, there was good agreement for aCL and aß2GPI IgG (Cohen’s kappa = 0.75 and 0.78, respectively) as well as for aCL and aß2GPI IgM (Cohen’s kappa = 0.64 and 0.65, respectively; Additional file 2: Table S2). Here, CD40LG is linked to autoimmune polyendocrinopathy.