GFP expression in EpCAM-positive or -negative cancer cell lines was evaluated, and both the EpCAM-positive human hepatocellular carcinoma cancer cell line, Huh7, and the EpCAM-negative hepatocellular carcinoma cancer cell line, SMMC-7721, exhibited GFP expression as early as 12 hours after oHSV1-hTERT-GFP transduction at Multiplicity of infection (MOI) = 1 (Figure 1B and 1C). Here, EPCAM is linked to infection.