At 48 h after transfection, the related expression level of PANDAR was determined by qRT-PCR and the results showed that the relative level of PANDAR in bladder cancer cells was significantly down-regulated by the PANDAR siRNA(Fig. 2a) and up-regulated by the pcDNA3.1-PANDAR (Fig. 2b). This evidence concerns the gene PANDAR and urinary bladder cancer.