Among these genes, we observed that Tfam contained high-affinity Hbp1-binding sites (P1: TTCATTCA and P2: GGGTAG(T)GG) at −9699 and −5098 bp from the transcriptional start, which are the well-characterized Hbp1 response elements.22, 23 The results of the chromatin immunoprecipitation (ChIP) analysis revealed the increased enrichment of Hbp1 binding to both sites in the Tfam promoter region in the ovaries of Hbp1-FH mice (Figure 3h). This evidence concerns the gene HBP1 and familial hyperaldosteronism.