To determine whether JNK1/2 mediates ITCH activation during 9F7-F11-induced HER3 ubiquitination and degradation in cancer cells, we analyzed by western blotting JNK1/2 (at Thr183/Tyr185) and ITCH (at Thr222) phosphorylation in BxPC3 (Figure 7A), DU145 (Figure 7B) and MDA-MB468 (Supplementary Figure S7) cancer cells following incubation with 9F7-F11 or/and NRG-1β, or medium alone (control). The gene discussed is ITCH; the disease is cancer.