To confirm the direct regulation of RelA expression by miR-7-5p in melanoma, we used quantitative real-time PCR (RT-qPCR) and western blotting and found that miR-7-5p reduces RelA mRNA (Figure 5A) and protein expression (Figure 5B) in WM266-4, A2058 and 1205Lu melanoma cells. Here, RELA is linked to melanoma.