In summary, by decreasing the expression of NR1, NR2B, and PKCε in spinal cord of IBS visceral hyperalgesia rats, moxibustion reduced the transduction of visceral hyperalgesia signals and inhibited the process of central sensitization, which might be an important mechanism by which moxibustion relieves the IBS visceral hyperalgesia status. Here, GRIN1 is linked to irritable bowel syndrome.