The methylation level of genes associated with autism spectrum disorder (Plxna4, Arid1b, Kdm4c, Runx1, Accn1, Aff2, Chd9, Cntnap2, Grip1, Grin2b, and Mid1); imprinted genes (Peg12, Tsix); transcriptional factors (Ebf2, Lmx1b, Runx3, Sox13, and Mef2a) that modulate neurogenesis; and genes related to neurodevelopment (Grik4, Ntrk2, Sgk1, Cacna1a, Gabrg3, Erbb3, and Gfra1) were found to be altered in CB of both male and female pups from the HMFA group. The gene discussed is TSIX; the disease is autism spectrum disorder.