Subgroup analysis based on cut-off value indicated that the effects of p16 on OS in patients with bladder cancer were associated with cut-off value (cut-off value ≤ 10%: HR = 1.83, 95% CI = 1.17~2.86, and P = 0.008; I2 = 3.2%, P = 0.006; cut-off value > 10%: HR = 1.40, 95% CI = 0.66~2.96, and P = 0.384; I2 = 0%, P = 0.951). The gene discussed is CDKN2A; the disease is urinary bladder carcinoma.