PLCG1 and cancer: To investigate whether 2-O-Bn-InsP5 inhibits cancer cell Matrigel invasion by specifically inhibiting PDK1/PLCγ1 pathway, we performed invasion assays using A375M cells transfected with anti-PLCγ1 specific siRNA (si-PLCγ1) or a non-targeting siRNA sequence (si-control) and treated with 2-O-Bn-InsP5 or with vehicle only (Fig. 5E).