TNFSF10 and cancer: Following validation of the stepwise tumorigenesis model, which included the determination of TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) sensitivity [16] (Additional file 2: Figure S2), we assessed the global gene expression in all three cell lines and the ratio of expression levels of immortalized to normal cells (BJEL/BJ) and cancer relative to normal cells (BJELM/BJ).