As shown in Fig. 2A,B, KD of OPN greatly reduced the tumor formation of grafted C6 cells as followed by T2WI measurement on days 12, 17 and 21 after implantation (ANOVA: F(1,48) = 98.1, p < 0.0001; Fisher’s post-hoc tests: p < 0.0001). The gene discussed is SPP1; the disease is neoplasm.