The relative expression levels of CASC2 were measured using qRT-PCR in 5 CRC cell lines and a total of 68 patients with CRC, normalized to GAPDH. CASC2 was significantly down-regulated in 76% (52/68) of CRC tissues compared with adjacent normal CRC tissues (P < 0.001, Fig. 1A,B). This evidence concerns the gene GAPDH and colorectal carcinoma.