The wild type CASC2 (psiCHECK2-CASC2-WT) and mutant CASC2 constructs (psiCHECK2-CASC2-MU) were cloned downstream of the luciferase gene (Fig. 3B) and transfected in CRC cells together with either miR-18a or miR-4735 mimics. This evidence concerns the gene CASC2 and colorectal carcinoma.