HEK-293E cells were transfected with HA-tagged wild-type (pcDNA-HA-ΔNp63) and Myc-tagged mutant (pcDNA-Myc-ΔNp63-R304W) constructs, as an in vitro cellular model of the EEC disease-related heterozygous status, and the five representative discriminatory siRNAs (T4, T11, T14, T15 and T16) at a concentration of 40 nM. The gene discussed is MYC; the disease is exstrophy-epispadias complex.