Moreover, the cytotoxicity testsof 4 and 8 toward human cervical cancer (HeLa) cells, human breastcancer (MCF–7) cells and mouse fibroblast (L929) cells were performed bythe 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay andthe cell apoptosis processes were characterized by calcein AM/PI stainingexperiments, annexin V-FITC/PI staining experiments and morphological changes. Here, ANXA5 is linked to cervical carcinoma.