Altogether these data showed that our syngenic orthotopic model in LysM-EGFP//CD11c-EYFP mice mimics GBM physiopathology (angiogenesis, invasiveness of parenchyma, necrotic core) and permits investigation of the changes in immunological parameters during GBM development by recurrent in vivo two- photon spectral imaging. Here, ITGAX is linked to glioblastoma.