This model employs the stereotaxic injection of a spheroid of fluorescent GL261 glioma cells stably expressing DsRed into the superficial layers of the parietal cortex (approximately 250 μm deep) of LysM-EGFP//CD11c-EYFP and occasionally of Thy1-CFP//LysM-EGFP//CD11c-EYFP mice to highlight subsets of cortical pyramidal neurons. The gene discussed is ITGAX; the disease is glioma.