To explore more widely the intrinsic immunopathogenic effect of AQP4-IgG and its functional and morphological consequence not only on astrocyte but also on myelin and axons and to evaluate its clinical impact, we have developed a new animal model of NMO based on a chronic infusion of “physiological dose” of purified immunoglobulins (IgG) from NMO patient and healthy individual, into the cerebrospinal fluid (CSF) of live rats. Here, AQP4 is linked to neuromyelitis optica.