Interestingly, the magnitude of responses to Env-CMDR was significantly higher four weeks after the second CN54rgp140/GLA-AF than at the time of the first CN54rgp140/GLA-AF immunization, median 100 SFC/million PBMCs (range 60–495) versus 85 (range 60–145) p<0.0001. The gene discussed is ERVW-1; the disease is atrial fibrillation.