Five days after stimulation, the T cells were used in a CTL assay using PC3 (human prostate carcinoma, MUC1+, HLA-A24+) as a target and ASPC-1 (human pancreatic carcinoma, MUC1+, HLA-A24NEG) as a negative control. The gene discussed is MUC1; the disease is exocrine pancreatic carcinoma.