We determined the genome-wide occupancy of WDR5 and using chromatin immunoprecipitation followed by deep-sequencing (ChIP-seq) in human RS4;11 ALL and THP-1 AML leukemia cells, we identified 1490 and 515 target genes for WDR5 in RS4;11 and THP-1 cells, respectively. Here, WDR5 is linked to acute myeloid leukemia.