HMGB1 and hepatocellular carcinoma: To confirm whether the loss of miR-129-2 is an important factor of HCC malignancy by upregulating HMGB1 expression, we performed that qRT-PCR and Western blot analysis and found that ectopic expression of miR-129-2 dramatically decreased the mRNA (Figure 4B) and protein (Figure 4C) expression of HMGB1.