ZIC5 mRNA was up-regulated 17 fold (p = 8.4E–07), ZIC2 8 fold (p = 1.3E–05) and HELLS 2 fold (p = 0.006) in Gleason grade 3 tumor glands derived from GS 4 + 3 = 7 as compared to GS 3 + 3 = 6 prostate cancer. This evidence concerns the gene ZIC5 and Familial prostate cancer.