To investigate the spatial relationship between viral infection and demyelination, using whole slide image segmentation we quantified the fractional area (termed the “stain index”) of each block’s white matter with positive VP1 (using the lower concentration of VP1 antibody to label only infected cells) and MBP IHC staining as estimates of viral cytopathic effect and extent of demyelination, respectively (Fig 6A). The gene discussed is MBP; the disease is viral infectious disease.