In conclusion, our results indicate that lncRNA DANCR promotes invasion and migration of prostate cancer cells in vitro and metastasis of tumor xenografts in nude mice, and decreases expression of TIMP2/3 synergistically with EZH2 through epigenetically silencing their promoter; moreover, DANCR expression is repressed by androgen-AR signaling pathway and DANCR knockdown facilitates the upregulation of TIMP2/3 and the suppression of invasion and migration by androgen-AR, while DANCR knockdown decreased the promotion of invasion and migration in prostate cancer cells by enzalutamide treatment. Here, AR is linked to Familial prostate cancer.