To substantiate the pathway analysis done by bioinformatics methods, we conducted immunohistochemistry (IHC) assays for three closely-related genes in the Wnt pathway, including SOX7, AXIN2 and β-catenin for 30 normal breast tissues and 74 breast cancer specimens with different pathological types, TNM (T: size or direct extent of the primary tumor; N: degree of spread to regional lymph nodes; M: presence of distant metastasis) stages and pathology grades. This evidence concerns the gene AXIN2 and breast cancer.