From the results of Western blot, we observed an efficient knockdown of PFN2, with over 50 % decrease in protein level in the PFN2-siRNA transfected ESCC cells, and the upregulated epithelial marker E-cadherin and significantly decreased mesenchymal markers: Vimentin, Snail, Slug and ZEB1 in Eca109, EC9706, and TE-1 cells with knockdown of PFN2 compared with the control groups (Fig. 5a, b, c, d). Here, ZEB1 is linked to esophageal squamous cell carcinoma.