The median survival time for patients with high PFN2 expression was 12 months (range, 1–96 months) and 42 months (range, 1–96 months) for patients with low PFN2 expression, which indicated that ESCC patients with high PFN2 expression had significantly shorter OS rates and greater risk of death than those with low PFN2 expression (log-rank test, χ2 = 5.203, P = 0.023) (Fig. 3a, b). This evidence concerns the gene PFN2 and esophageal squamous cell carcinoma.